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goat anti rabbit biotinylated secondary antibody  (Jackson Immuno)


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    Structured Review

    Jackson Immuno goat anti rabbit biotinylated secondary antibody
    Goat Anti Rabbit Biotinylated Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/igg+fc/Biotin-SP+(long+spacer)+AffiniPure+F(ab')%E2%82%82+Fragment+Goat+Anti-Rabbit+IgG%2C+Fc+fragment+specific/pmc12996694-111-14-22
    Average 93 stars, based on 34 article reviews
    goat anti rabbit biotinylated secondary antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Effects of adjuvants in a rabies-vectored Ebola virus vaccine on protection from surrogate challenge
    Article Snippet: .. Plates were kept at 4 °C overnight, washed, and incubated for 2 hr at RT with 100 μL per well horseradish peroxidase-conjugated goat anti-mouse IgG (H + L) (Jackson ImmunoResearch, Cat# 115-035-003), IgG-Fc (Cat# 115-005-008), IgG2c (Cat# 115-035-208), or IgG1 (Cat# 115-035-205) antibody diluted in PBST 1:20,000. ..

    Article Title: Pemphigus Vulgaris Autoantibody Profiling by Proteomic Technique
    Article Snippet: .. The slides were washed five times in 10 mM Tris (pH 8.0)-150 mM NaCl containing 0.05% Tween 20 buffer, and bound antibodies were detected after 1 h incubation in Biotin-SP conjugated affinity purified human goat secondary antibody to IgG Fc (Fc-γ fragment specific) (Jackson Immunoresearch, West Grove, PA) diluted 1/200 in blocking buffer. .. The slides were washed three times, and bound antibodies detected by 1 h incubation with StreptAvidin conjugated with the dye PBXL-3 as tertiary antibody (streptavidin-conjugated SureLight® P-3) (Columbia Biosciences Corporation, Columbia, MD) diluted 1/200 in blocking buffer.

    Article Title: Polypeptide
    Article Snippet: .. Biotinylated IgG1κ (at a concentration of 0.05 and 0.5 μg/ml for clones derived from round 4 and 0.01 μg/ml for clones from round 5) or IgG Fc (at a concentration of 0.5 μg/ml; Jackson Immunoresearch, cat. no. 009-008, lot 66321) in 100 μl PBS-T0.05 was added to the wells and incubated for 1.5 h. Bound target was detected with SA-HRP (Dako, cat. no. P0397), diluted 1:5000 in PBS-T0.05, and incubated for 1 h at room temperature. ..

    Article Title: TPP-45142—an Anti-HER2 T-cell Engager—Designed for Selective HER2-Low Cancer Immunotherapy
    Article Snippet: For detection of HER2 expression in endothelial cells, EA. hy926 cells were harvested, washed using PBS, and stained using Live-Dead Aqua (Invitrogen) for 20 minutes on ice. .. After washing and blocking using IgG-Fc (mouse normal serum, Jackson ImmunoResearch Inc. cat. #015-000-120, RRID: AB_2337194), the cells were incubated with anti-HER2/neu FITC antibody (BD Biosciences, cat. #340880, RRID: AB_400166). .. Thereafter, the cells were washed with buffered medium and analyzed on a BD Fortessa flow cytometer (BD Biosciences) equipped with BD FACS Diva software (version 9).

    Affinity Purification:

    Article Title: Pemphigus Vulgaris Autoantibody Profiling by Proteomic Technique
    Article Snippet: .. The slides were washed five times in 10 mM Tris (pH 8.0)-150 mM NaCl containing 0.05% Tween 20 buffer, and bound antibodies were detected after 1 h incubation in Biotin-SP conjugated affinity purified human goat secondary antibody to IgG Fc (Fc-γ fragment specific) (Jackson Immunoresearch, West Grove, PA) diluted 1/200 in blocking buffer. .. The slides were washed three times, and bound antibodies detected by 1 h incubation with StreptAvidin conjugated with the dye PBXL-3 as tertiary antibody (streptavidin-conjugated SureLight® P-3) (Columbia Biosciences Corporation, Columbia, MD) diluted 1/200 in blocking buffer.

    Blocking Assay:

    Article Title: Pemphigus Vulgaris Autoantibody Profiling by Proteomic Technique
    Article Snippet: .. The slides were washed five times in 10 mM Tris (pH 8.0)-150 mM NaCl containing 0.05% Tween 20 buffer, and bound antibodies were detected after 1 h incubation in Biotin-SP conjugated affinity purified human goat secondary antibody to IgG Fc (Fc-γ fragment specific) (Jackson Immunoresearch, West Grove, PA) diluted 1/200 in blocking buffer. .. The slides were washed three times, and bound antibodies detected by 1 h incubation with StreptAvidin conjugated with the dye PBXL-3 as tertiary antibody (streptavidin-conjugated SureLight® P-3) (Columbia Biosciences Corporation, Columbia, MD) diluted 1/200 in blocking buffer.

    Article Title: TPP-45142—an Anti-HER2 T-cell Engager—Designed for Selective HER2-Low Cancer Immunotherapy
    Article Snippet: For detection of HER2 expression in endothelial cells, EA. hy926 cells were harvested, washed using PBS, and stained using Live-Dead Aqua (Invitrogen) for 20 minutes on ice. .. After washing and blocking using IgG-Fc (mouse normal serum, Jackson ImmunoResearch Inc. cat. #015-000-120, RRID: AB_2337194), the cells were incubated with anti-HER2/neu FITC antibody (BD Biosciences, cat. #340880, RRID: AB_400166). .. Thereafter, the cells were washed with buffered medium and analyzed on a BD Fortessa flow cytometer (BD Biosciences) equipped with BD FACS Diva software (version 9).

    Concentration Assay:

    Article Title: Polypeptide
    Article Snippet: .. Biotinylated IgG1κ (at a concentration of 0.05 and 0.5 μg/ml for clones derived from round 4 and 0.01 μg/ml for clones from round 5) or IgG Fc (at a concentration of 0.5 μg/ml; Jackson Immunoresearch, cat. no. 009-008, lot 66321) in 100 μl PBS-T0.05 was added to the wells and incubated for 1.5 h. Bound target was detected with SA-HRP (Dako, cat. no. P0397), diluted 1:5000 in PBS-T0.05, and incubated for 1 h at room temperature. ..

    Clone Assay:

    Article Title: Polypeptide
    Article Snippet: .. Biotinylated IgG1κ (at a concentration of 0.05 and 0.5 μg/ml for clones derived from round 4 and 0.01 μg/ml for clones from round 5) or IgG Fc (at a concentration of 0.5 μg/ml; Jackson Immunoresearch, cat. no. 009-008, lot 66321) in 100 μl PBS-T0.05 was added to the wells and incubated for 1.5 h. Bound target was detected with SA-HRP (Dako, cat. no. P0397), diluted 1:5000 in PBS-T0.05, and incubated for 1 h at room temperature. ..

    Derivative Assay:

    Article Title: Polypeptide
    Article Snippet: .. Biotinylated IgG1κ (at a concentration of 0.05 and 0.5 μg/ml for clones derived from round 4 and 0.01 μg/ml for clones from round 5) or IgG Fc (at a concentration of 0.5 μg/ml; Jackson Immunoresearch, cat. no. 009-008, lot 66321) in 100 μl PBS-T0.05 was added to the wells and incubated for 1.5 h. Bound target was detected with SA-HRP (Dako, cat. no. P0397), diluted 1:5000 in PBS-T0.05, and incubated for 1 h at room temperature. ..



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    Jackson Immuno goat anti rabbit biotinylated secondary antibody
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    Jackson Immuno biotinylated goat anti rabbit
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    Image Search Results


    Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).

    Journal: Journal of Translational Autoimmunity

    Article Title: Anti-IgLON5 encephalitis is associated with anti-retinal immunological reactivity without retinal alteration

    doi: 10.1016/j.jtauto.2026.100359

    Figure Lengend Snippet: Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).

    Article Snippet: The reactivity of the patients’ sera and/or CSF against the retina was evaluated retrospectively with frozen samples (−80°, EXPLAINEUR biobank) through an indirect immunofluorescence technique using sections of monkey retina (Ref. FA1172-1005, Euroimmun), detected with an FITC-labelled secondary antibody anti-human IgAGM (Euroimmun conjugate) or directed against IgA (ref. F0204, DAKO), IgM (ref. F0203, DAKO), IgG1 (ref. 9052-02, Southern Biotech) and IgG4 (ref. 9200-02, Southern Biotech).

    Techniques: Immunofluorescence, Fluorescence, Control